negative inference; confirmed by additional inferences
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EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p562, left bottom
"Of the PV[parvalbumin]-immunoreactive cells analyzed, 88.5% were dis-
tinctly colocalized with the M2AchR [muscarinic 2 acetylcholine receptor] protein (Figs. 2 and 3).
The remaining cells (11.5%) belonged to the cate-
gory of unclassi?ed cells where the presence of M2AchR
could not be unequivocally determined. None of the
PV-immunoreactive cells were clearly negative for
M2AchR. The colocalization pattern was relatively uniform
in layers II-VI. In layer I, there were no PV-immunoreactive
cells. No differences in colocalization patterns were observed
across the different EC subfields."
Interpretation notes: Glutamatergic and GABAergic types in EC I are negative for PV
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p188, left bot
"These [entorhinal cortex] sections showed an abundance of cells expressing only one of the markers [parvalbumin or calretinin]. Cells in which the two immunofluorescent labels coexisted were conspicuously absent. These results indicate that there is a complete segregation of populations of entorhinal parvalbumin- and calretinin-immunoreactive cells."
Interpretation notes: Glutamatergic and GABAergic CR+ types in EC I_II_III_IV_V_VI are probably PV-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p188, left bot
"These [entorhinal cortex] sections showed an abundance of cells expressing only one of the markers [parvalbumin or calretinin]. Cells in which the two immunofluorescent labels coexisted were conspicuously absent. These results indicate that there is a complete segregation of populations of entorhinal parvalbumin- and calretinin-immunoreactive cells."
Interpretation notes: Glutamatergic and GABAergic CR+ types in EC I_II_III_IV_V_VI are probably PV- by contrapositive logic
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p116, Table 1
"Table 1 Numbers of GABA- and GAD-positive parvalbumin (PV) immunoreactive neurons in the different layers of the medial and lateral entorhinal cortex (EC)"
Interpretation notes: Glutamatergic and GABAergic types in EC I are negative for PV
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negative inference; confirmed by additional inferences
