negative inference; confirmed by additional inferences
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EXPRESSION: negative (inference)
ANIMAL: mouse
PROTOCOL: mRNA
Page location: p1525, left bottom
"In situ hybridization for Som [somatostatin] combined with immunohistochemistry for Tomato [acetylcholine receptor alpha 2 subunit-promoter]
revealed that the vast majority (95.1%, 214/225 cells) of Tomato+ cells
were also Som+ and comprised a subpopulation (35.2%, 214 of 608
neurons) of CA1 Som+ interneurons (Supplementary Fig. 1c,d)."
Interpretation notes: Glutamatergic and GABAergic SOM- types in CA1 SLM_SR_SP_SO are probably Chrna2- by contrapositive logic; Clopper-Pearson confidence interval lower bound = 91.4% (n = 214/225)
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 559 left bottom
"We tested CR [calretinin] and CCK[cholecystokinin]-immunolabelling in VGLUT3[vesicular glutamate transporter type 3]-positive somata in the CA1 area. Somatic CCK- or CR- labelling occurred alone in 32.7% and 54.8% of the cells, respectively. There was no colocalization between CR and VGLUT3, or CR and CCK (Fig. 7B and Table 2). Likewise, none of the tested VGLUT3-positive cells in the CA3 area/dentate gyrus (123 cells) and isocortex (40 cells) was immunoreactive for CR."
Interpretation notes: Glutamatergic and GABAergic CCK+ types in CA1 SLM_SR_SP_SO are probably CR-; Clopper-Pearson confidence interval lower bound = 97.1% (n = 126/126)
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 559 left bottom
"We tested CR [calretinin] and CCK[cholecystokinin]-immunolabelling in VGLUT3[vesicular glutamate transporter type 3]-positive somata in the CA1 area. Somatic CCK- or CR- labelling occurred alone in 32.7% and 54.8% of the cells, respectively. There was no colocalization between CR and VGLUT3, or CR and CCK (Fig. 7B and Table 2). Likewise, none of the tested VGLUT3-positive cells in the CA3 area/dentate gyrus (123 cells) and isocortex (40 cells) was immunoreactive for CR."
Interpretation notes: Glutamatergic and GABAergic CCK+ types in CA1 SLM_SR_SP_SO are probably CR- by contrapositive logic
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negative inference; confirmed by additional inferences