negative inference; confirmed by additional inferences
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EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p311, right middle
"With the exception of one [calretinin and calbindin] double-labeled cell in the molecular layer of the dentate gyrus in one of the animals (at the temporal pole of the hippocampus), all double-labeled cells were present in specific topographical locations. The first of these was the alveus and stratum oriensof field CA1, where we observed most of the double-labeled cells (Figs. 1 and 2AB). Whereas the entire extent of the alveus and stratum oriens of field CA1 contained double-labeled cells, the largest concentration of these cells was observed in the temporal part of CA1. The second location was the small area of transition at the border between field CA1 and the subiculum where the typical palisade organization of the stratum pyramidale of field CA1 is lost and over a short trajectory becomes the loosely organized cell layer typical of the subiculum (Fig.2C, detail in Fig. 2D). Most double-labeled cells in this region were present in the temporal hippocampus). The third location of double-labeled cells was in the fimbria. Here we found some scattered double-labeled cells (Fig.1). Apart from these hippocampal locations we noted double-labeled cells in the deep layer of the ventral subiculum and some double-labeled cells in the deep layers(V-VI) of the entorhinal cortex."
Interpretation notes: Glutamatergic and GABAergic CR+ types in CA1 SLM_SR_SP are probably CB-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p311, right middle
"With the exception of one [calretinin and calbindin] double-labeled cell in the molecular layer of the dentate gyrus in one of the animals (at the temporal pole of the hippocampus), all double-labeled cells were present in specific topographical locations. The first of these was the alveus and stratum oriensof field CA1, where we observed most of the double-labeled cells (Figs. 1 and 2AB). Whereas the entire extent of the alveus and stratum oriens of field CA1 contained double-labeled cells, the largest concentration of these cells was observed in the temporal part of CA1. The second location was the small area of transition at the border between field CA1 and the subiculum where the typical palisade organization of the stratum pyramidale of field CA1 is lost and over a short trajectory becomes the loosely organized cell layer typical of the subiculum (Fig.2C, detail in Fig. 2D). Most double-labeled cells in this region were present in the temporal hippocampus). The third location of double-labeled cells was in the fimbria. Here we found some scattered double-labeled cells (Fig.1). Apart from these hippocampal locations we noted double-labeled cells in the deep layer of the ventral subiculum and some double-labeled cells in the deep layers(V-VI) of the entorhinal cortex."
Interpretation notes: Glutamatergic and GABAergic CR+ types in CA1 SLM_SR_SP are probably CB- by contrapositive logic
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 312, left top
Linking PMID: 8783251 Linking Quote: All necessary morphological evidence is present in this self-same publication.
"CCK [cholecystokinin] was co-localized with VIP [vasoactive intestinal polypeptide] in basket cells, but was absent from the other types (except for a few cells with the axon in stratum radiatum and dendrites in all layers.) Calbindin and parvalbumin showed no coexistence with VIP. Co-localization with GABA, revealed 100% coexistence with VIP."
Interpretation notes: Glutamatergic and GABAergic VIP+ types in CA1 SLM_SR_SP_SO are probably CB-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 311, right middle
"From 210 VIP-immunoreactive interneurons in CA1, 208 were negative for calbindin D-28k (Fig. 8C, D) whereas none of the 44 VIP-containing neurons in CA3 showed calbindin D-28k immunofluorescence."
Interpretation notes: Glutamatergic and GABAergic VIP+ types in CA1 SLM_SR_SP_SO are probably CB-; Clopper-Pearson confidence interval lower bound = 96.6% (n = 208/210)
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negative inference; confirmed by additional inferences