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EXPRESSION: positive (inference)
ANIMAL: mouse
PROTOCOL: mRNA
Page location: Supplementary p3, Fig. 2
"Supplementary Figure 2. Viaat [vesicular inhibitory amino acid transporter] mRNA is selectively lost in OLM?2 [acetylcholine receptor alpha 2 subunit expressing oriens-lacunosum moleculare] cells in Chrna2-cre/Viaat lx
mice. Fluorescent photomicrographs of horizontal hippocampal sections of Chrna2-cre/R26 tom animals
obtained by laser-scanning confocal microscopy. Chrna2-cre activity and Viaat mRNA expression in
cell bodies were visualized by combined immunofluorescence for Red Fluorescent Protein (RFP, red)
to detect tdTomato expressing cells and fluorescent in situ hybridization for Viaat (green). (a) All
Chrna2-cre positive cells also expressed Viaat mRNA in sections from Chrna2-cre/R26 tom mice
(n=103). Arrowheads indicate cells positive for both Viaat mRNA and Chrna2-cre activity."
Interpretation notes: Glutamatergic and GABAergic Chrna2+ types in CA1 SLM_SR_SP_SO are probably VGAT+; Clopper-Pearson confidence interval lower bound = 96.5% (n = 103/103)
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positive inference
