negative inference; confirmed by additional inferences
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EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 559 left bottom
"We tested CR [calretinin] and CCK[cholecystokinin]-immunolabelling in VGLUT3[vesicular glutamate transporter type 3]-positive somata in the CA1 area. Somatic CCK- or CR- labelling occurred alone in 32.7% and 54.8% of the cells, respectively. There was no colocalization between CR and VGLUT3, or CR and CCK (Fig. 7B and Table 2). Likewise, none of the tested VGLUT3-positive cells in the CA3 area/dentate gyrus (123 cells) and isocortex (40 cells) was immunoreactive for CR."
Interpretation notes: Glutamatergic and GABAergic CR+ types in CA1 SLM_SR_SP_SO are probably vGlut3-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p562, left mid
"The lack of PV [parvalbumin] immunoreactivity in VGLUT3[vesicular glutamate transporter type 3]-positive somata and boutons, the obligatory coexpression of VGLUT3 and CCK [cholecystokinin] in interneuronal somata and the perisomatic location of boutons double-labelled for GAD and VGLUT3 in the principal cell layers demonstrate that VGLUT3 is present in a distinct type of basket cell. Some basket cells expressing CCK but negative for PV also express VIP [vasoactive intestinal polypeptide] (reviewed in Freund & Buzsaki, 1996). However, in our material, neither VIP-positive cell bodies nor boutons were positive for VGLUT3. Therefore, the CCK- and VGLUT3-positive cells constitute a novel subtype of basket cell {CA1 Basket CCK+}."
Interpretation notes: Glutamatergic and GABAergic VIP+ types in CA1 SLM_SR_SP_SO are probably vGlut3-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p562, left mid
"The lack of PV [parvalbumin] immunoreactivity in VGLUT3[vesicular glutamate transporter type 3]-positive somata and boutons, the obligatory coexpression of VGLUT3 and CCK [cholecystokinin] in interneuronal somata and the perisomatic location of boutons double-labelled for GAD and VGLUT3 in the principal cell layers demonstrate that VGLUT3 is present in a distinct type of basket cell. Some basket cells expressing CCK but negative for PV also express VIP [vasoactive intestinal polypeptide] (reviewed in Freund & Buzsaki, 1996). However, in our material, neither VIP-positive cell bodies nor boutons were positive for VGLUT3. Therefore, the CCK- and VGLUT3-positive cells constitute a novel subtype of basket cell {CA1 Basket CCK+}."
Interpretation notes: Glutamatergic and GABAergic CCK- types in CA1 SLM_SR_SP_SO are probably vGlut3- by contrapositive logic
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 559 left bottom
"We tested CR [calretinin] and CCK[cholecystokinin]-immunolabelling in VGLUT3[vesicular glutamate transporter type 3]-positive somata in the CA1 area. Somatic CCK- or CR- labelling occurred alone in 32.7% and 54.8% of the cells, respectively. There was no colocalization between CR and VGLUT3, or CR and CCK (Fig. 7B and Table 2). Likewise, none of the tested VGLUT3-positive cells in the CA3 area/dentate gyrus (123 cells) and isocortex (40 cells) was immunoreactive for CR."
Interpretation notes: Glutamatergic and GABAergic CR+ types in CA1 SLM_SR_SP_SO are probably vGluT3- by contrapositive logic
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 559 left middle
"All VGLUT3-positive somata [in CA1] were negative for VIP, but 10.7 +/- 2.5% of CCK-positive cells were also positive for VIP (Fig. 7A and Table 2). This figure is similar to that reported by Kosaka et al. (1985). In these reactions more than 20% of CCK-positive interneurons was also VGLUT3-positive in their perikarya (Table 2). None of the tested VGLUT3-positive neurons in the CA3 area/dentate gyrus (54 cells) and isocortex (24 cells) was immunoreactive for VIP."
Interpretation notes: Glutamatergic and GABAergic VIP+ types in CA1 SLM_SR_SP_SO are probably vGluT3- by contrapositive logic
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p562, left mid
"The lack of PV [parvalbumin] immunoreactivity in VGLUT3[vesicular glutamate transporter type 3]-positive somata and boutons, the obligatory coexpression of VGLUT3 and CCK [cholecystokinin] in interneuronal somata and the perisomatic location of boutons double-labelled for GAD and VGLUT3 in the principal cell layers demonstrate that VGLUT3 is present in a distinct type of basket cell. Some basket cells expressing CCK but negative for PV also express VIP [vasoactive intestinal polypeptide] (reviewed in Freund & Buzsaki, 1996). However, in our material, neither VIP-positive cell bodies nor boutons were positive for VGLUT3. Therefore, the CCK- and VGLUT3-positive cells constitute a novel subtype of basket cell {CA1 Basket CCK+}."
Interpretation notes: Glutamatergic and GABAergic VIP+ types in CA1 SLM_SR_SP_SO are probably vGlut3- by contrapositive logic
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negative inference; confirmed by additional inferences