negative inference; confirmed by additional inferences
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EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p311, right middle
"With the exception of one [calretinin and calbindin] double-labeled cell in the molecular layer of the dentate gyrus in one of the animals (at the temporal pole of the hippocampus), all double-labeled cells were present in specific topographical locations. The first of these was the alveus and stratum oriensof field CA1, where we observed most of the double-labeled cells (Figs. 1 and 2AB). Whereas the entire extent of the alveus and stratum oriens of field CA1 contained double-labeled cells, the largest concentration of these cells was observed in the temporal part of CA1. The second location was the small area of transition at the border between field CA1 and the subiculum where the typical palisade organization of the stratum pyramidale of field CA1 is lost and over a short trajectory becomes the loosely organized cell layer typical of the subiculum (Fig.2C, detail in Fig. 2D). Most double-labeled cells in this region were present in the temporal hippocampus). The third location of double-labeled cells was in the fimbria. Here we found some scattered double-labeled cells (Fig.1). Apart from these hippocampal locations we noted double-labeled cells in the deep layer of the ventral subiculum and some double-labeled cells in the deep layers(V-VI) of the entorhinal cortex."
Interpretation notes: Glutamatergic and GABAergic CR+ types in DG Smi_Smo_SG_H are probably CB-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p311, right middle
"With the exception of one [calretinin and calbindin] double-labeled cell in the molecular layer of the dentate gyrus in one of the animals (at the temporal pole of the hippocampus), all double-labeled cells were present in specific topographical locations. The first of these was the alveus and stratum oriensof field CA1, where we observed most of the double-labeled cells (Figs. 1 and 2AB). Whereas the entire extent of the alveus and stratum oriens of field CA1 contained double-labeled cells, the largest concentration of these cells was observed in the temporal part of CA1. The second location was the small area of transition at the border between field CA1 and the subiculum where the typical palisade organization of the stratum pyramidale of field CA1 is lost and over a short trajectory becomes the loosely organized cell layer typical of the subiculum (Fig.2C, detail in Fig. 2D). Most double-labeled cells in this region were present in the temporal hippocampus). The third location of double-labeled cells was in the fimbria. Here we found some scattered double-labeled cells (Fig.1). Apart from these hippocampal locations we noted double-labeled cells in the deep layer of the ventral subiculum and some double-labeled cells in the deep layers(V-VI) of the entorhinal cortex."
Interpretation notes: Glutamatergic and GABAergic CR+ types in DG Smi_Smo_SG_H are probably CB- by contrapositive logic
EXPRESSION: negative (inference)
ANIMAL: mouse
PROTOCOL: immunohistochemistry
Page location: p49, Table 2
"Table 2. Co-localization between neuropeptides and calcium-binding proteins"
Interpretation notes: Glutamatergic and GABAergic SOM+ types in DG H are probably CB-; Clopper-Pearson confidence interval lower bound = 85.2% (n = 23/23)
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p 193 left
Linking PMID: 2419523 Linking Quote: Citation included in evidentiary quote.
"Conversely, the numerous and distinct populations of hilar somatostatin-immunoreactive cells and hilar mossy cells identified in previous studies (Ribak et al., '85; Sloviter and Nilaver, '87) do not exhibit PV-LI [parvalbumin-like immunoreactivity] or CaBP-LI [calbindin D(28k)-like immunoreactivity]."
Interpretation notes: Glutamatergic and GABAergic SOM+ types in DG H are probably CB-
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negative inference; confirmed by additional inferences
