negative inference; confirmed by additional inferences
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EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p245, left bottom
"The VGLUT3-, CCK+ subpopulation of basket cells also project to the cell bodies and proximal dendrites of the granule cells (Boulland et al. 2004; Somogyi et al. 2004). However, hippocampal sections triple stained for CCK, VGLUT2, and VGAT did not show triple colocalization (Fig. 5C). CCK and VGAT colocalized in a subset of terminals (bright blue, double arrowheads; right inset), but terminals containing both
VGLUT2 and VGAT (yellow, single arrowheads; left inset) were not stained for CCK. The VGLUT2+ terminals therefore do not belong to the VGLUT3 or CCK expressing populations of interneurons."
Interpretation notes: Glutamatergic and GABAergic CCK+ types in DG Smo_Smi_SG_H are probably vGlut2-
EXPRESSION: negative (inference)
ANIMAL: rat
PROTOCOL: immunohistochemistry
Page location: p245, left bottom
"The VGLUT3-, CCK+ subpopulation of basket cells also project to the cell bodies and proximal dendrites of the granule cells (Boulland et al. 2004; Somogyi et al. 2004). However, hippocampal sections triple stained for CCK, VGLUT2, and VGAT did not show triple colocalization (Fig. 5C). CCK and VGAT colocalized in a subset of terminals (bright blue, double arrowheads; right inset), but terminals containing both
VGLUT2 and VGAT (yellow, single arrowheads; left inset) were not stained for CCK. The VGLUT2+ terminals therefore do not belong to the VGLUT3 or CCK expressing populations of interneurons."
Interpretation notes: Glutamatergic and GABAergic CCK+ types in DG Smo_Smi_SG_H are probably vGlut2- by contrapositive logic
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negative inference; confirmed by additional inferences